Publications (recent, selected)

Acute lung injury

Tissue-Resident Alveolar Macrophages Do Not Rely on Glycolysis for LPS-induced Inflammation

Parker S Woods, Lucas M Kimmig, Angelo Y Meliton, Kaitlyn A Sun, Yufeng Tian, Erin M O’Leary, Gizem A Gökalp, Robert B Hamanaka, Gökhan M Mutlu. American Journal of Respiratory Cell and Molecular Biology. 2020 Feb;62(2):243-255. doi: 10.1165/rcmb.2019-0244OC

Experimental Lung Injury Reduces Krüppel-like Factor 2 to Increase Endothelial Permeability via Regulation of RAPGEF3-Rac1 Signaling.

Huang RT, Wu D, Meliton A, Oh MJ, Krause M, Lloyd JA, Nigdelioglu R, Hamanaka RB, Jain MK, Birukova A, Kress JP, Birukov KG, Mutlu GM, Fang Y. American Journal of Respiratory and Critical Care Medicine. 2017 Mar 1;195(5):639-651.doi: 10.1164/rccm.201604-0668OC

SARS-CoV-2 Infection is Associated with Reduced Krüppel-Like Factor 2 in Human Lung Autopsy.

Wu D, Lee TH, Huang RT, Guzy R, Schoettler N, Adegunsoye A, Mueller J, Husain A, Sperling A, Mutlu GM, Fang Y.Am J Respir Cell Mol Biol. 2021 May 10. doi: 10.1165/rcmb.2020-0564LE. Online ahead of print.PMID: 33971111

Association of Vitamin D Status and Other Clinical Characteristics With COVID-19 Test Results.

Meltzer DO, Best TJ, Zhang H, Vokes T, Arora V, Solway J.JAMA Netw Open. 2020 Sep 1;3(9):e2019722. doi: 10.1001/jamanetworkopen.2020.19722.PMID: 32880651

IL-33-mediated Eosinophilia Protects against Acute Lung Injury.Krishack PA, Hollinger MK, Kuzel TG, Decker TS, Louviere TJ, Hrusch CL, Sperling AI, Verhoef PA.Am J Respir Cell Mol Biol. 2021 May;64(5):569-578. doi: 10.1165/rcmb.2020-0166OC.PMID: 33571420

Basic Sciences

Targeted polyelectrolyte complex micelles treat vascular complications in vivo.
Zhengjie Zhou, Chih-Fan Yeh, Michael Mellas, Myung-Jin Oh, Jiayu Zhu, Jin Li, Ru-Ting Huang, Devin Harrison, Tzu-Pin Shentu, David Wu, Michael Lueckheide, Lauryn Carver, Eun Ji Chung, Lorraine Leon, Kai-Chien Yang, Matthew V. Tirrell*, Yun Fang*, Targeted polyelectrolyte complex micelles treat vascular complications in vivo, PNAS, 2021. 10.1073/pnas.2114842118.

Jin Li, Yun Fang* and David Wu*. Mechanical forces and metabolic changes cooperate to drive cellular memory and endothelial phenotypes. Current Topics in Membranes: Cellular Mechanostransduction Mechanisms to Cardiovascular and Fibrotic Diseases. Accepted. 2021.

Single-cell lactate production rate as a measure of glycolysis in endothelial cells.
Devin Harrison*, David Wu*, Jun Huang, Yun Fang. STAR Protocols. 2021. doi: 10.1016/j.xpro.2021.100807 *equivalent contribution (co-first authorship)

Single-cell metabolic imaging reveals a SLC2A3-dependent glycolytic burst in motile

endothelial cells
David Wu, Devin L. Harrison, Teodora Szasz, Chih-Fan Yeh, Tzu-Pin Shentu, Angelo Meliton, Ru-Ting Huang, Zhengjie Zhou, Gökhan M. Mutlu, Jun Huang & Yun Fang. Nature Metabolism 3, 714–727 (2021). https://doi.org/10.1038/s42255-021-00390-y

Experimental Lung Injury Reduces Krüppel-like Factor 2 to Increase Endothelial Permeability via Regulation of RAPGEF3-Rac1 Signaling.

HIF-1α is required for disturbed flow-induced metabolic reprogramming in human and porcine vascular endothelium.

Wu D, Huang RT, Hamanaka RB, Krause M, Oh MJ, Kuo CH, Nigdelioglu R, Meliton AY, Witt L, Dai G, Civelek M, Prabhakar NR, Fang Y, Mutlu GM.Elife. 2017 May 30;6:e25217. doi: 10.7554/eLife.25217.PMID: 28556776

Asthma

T-cell phenotypes are associated with serum IgE levels in Amish and Hutterite children.

Hrusch CL, Stein MM, Gozdz J, Holbreich M, von Mutius E, Vercelli D, Ober C, Sperling AI.J Allergy Clin Immunol. 2019 Nov;144(5):1391-1401.e10. doi: 10.1016/j.jaci.2019.07.034. Epub 2019 Aug 8.PMID: 31401285

Shared and distinct genetic risk factors for childhood-onset and adult-onset asthma: genome-wide and transcriptome-wide studies.

Pividori M, Schoettler N, Nicolae DL, Ober C, Im HK.Lancet Respir Med. 2019 Jun;7(6):509-522. doi: 10.1016/S2213-2600(19)30055-4. Epub 2019 Apr 27.PMID: 31036433

Distinct associations of sputum and oral microbiota with atopic, immunologic, and clinical features in mild asthma.

Durack J, Christian LS, Nariya S, Gonzalez J, Bhakta NR, Ansel KM, Beigelman A, Castro M, Dyer AM, Israel E, Kraft M, Martin RJ, Mauger DT, Peters SP, Rosenberg SR, Sorkness CA, Wechsler ME, Wenzel SE, White SR, Lynch SV, Boushey HA, Huang YJ; National Heart, Lung, and Blood Institute’s ”AsthmaNet“.J Allergy Clin Immunol. 2020 Nov;146(5):1016-1026. doi: 10.1016/j.jaci.2020.03.028. Epub 2020 Apr 13.PMID: 32298699

Critical Care Outcomes

Alternatives to Invasive Ventilation in the COVID-19 Pandemic.

Patel BK, Kress JP, Hall JB.JAMA. 2020 Jul 7;324(1):43-44. doi: 10.1001/jama.2020.9611.PMID: 32496506

ICU mobility and improved outcomes: still searching for the Holy Grail.

Stutz MR, Kress JP, Wolfe KS.Thorax. 2021 May 13:thoraxjnl-2021-217009. doi: 10.1136/thoraxjnl-2021-217009. Online ahead of print.PMID: 33986145

Effect of Noninvasive Ventilation Delivered by Helmet vs Face Mask on the Rate of Endotracheal Intubation in Patients With Acute Respiratory Distress Syndrome: A Randomized Clinical Trial.

Patel BK, Wolfe KS, Pohlman AS, Hall JB, Kress JP.JAMA. 2016 Jun 14;315(22):2435-41. doi: 10.1001/jama.2016.6338.PMID: 27179847

Impact of Vasoactive Medications on ICU-Acquired Weakness in Mechanically Ventilated Patients.

Wolfe KS, Patel BK, MacKenzie EL, Giovanni SP, Pohlman AS, Churpek MM, Hall JB, Kress JP.Chest. 2018 Oct;154(4):781-787. doi: 10.1016/j.chest.2018.07.016. Epub 2018 Sep 11.PMID: 30217640

Fibrosis

Anti-fibrotic effects of tannic acid through regulation of a sustained TGF-beta receptor signaling.

Reed EB, Ard S, La J, Park CY, Culligan L, Fredberg JJ, Smolyaninova LV, Orlov SN, Chen B, Guzy R, Mutlu GM, Dulin NO.Respir Res. 2019 Jul 29;20(1):168. doi: 10.1186/s12931-019-1141-8.

Calcium-Activated Chloride Channel ANO1/TMEM16A: Regulation of Expression and Signaling.

Dulin NO.Front Physiol. 2020 Nov 5;11:590262. doi: 10.3389/fphys.2020.590262. eCollection 2020.PMID: 33250781

Fibroblast-enriched endoplasmic reticulum protein TXNDC5 promotes pulmonary fibrosis by augmenting TGFβ signaling through TGFBR1 stabilization.

Lee TH, Yeh CF, Lee YT, Shih YC, Chen YT, Hung CT, You MY, Wu PC, Shentu TP, Huang RT, Lin YS, Wu YF, Lin SJ, Lu FL, Tsao PN, Lin TH, Lo SC, Tseng YS, Wu WL, Chen CN, Wu CC, Lin SL, Sperling AI, Guzy RD, Fang Y, Yang KC.Nat Commun. 2020 Aug 26;11(1):4254. doi: 10.1038/s41467-020-18047-x.PMID: 32848143

FGFR2 Is Required for AEC2 Homeostasis and Survival after Bleomycin-induced Lung Injury.

Dorry SJ, Ansbro BO, Ornitz DM, Mutlu GM, Guzy RD.Am J Respir Cell Mol Biol. 2020 May;62(5):608-621. doi: 10.1165/rcmb.2019-0079OC.PMID: 31860803 Free PMC article.

Glutamine Metabolism Is Required for Collagen Protein Synthesis in Lung Fibroblasts.

Hamanaka RB, O’Leary EM, Witt LJ, Tian Y, Gökalp GA, Meliton AY, Dulin NO, Mutlu GM.Am J Respir Cell Mol Biol. 2019 Nov;61(5):597-606. doi: 10.1165/rcmb.2019-0008OC.PMID: 30973753

Molecular pathways in idiopathic pulmonary fibrosis pathogenesis: Transcending barriers to optimally targeted pharmacotherapies.

Strykowski R, Adegunsoye A.EBioMedicine. 2021 May 6;67:103373. doi: 10.1016/j.ebiom.2021.103373. Online ahead of print.PMID: 33965873

Chung JH, Adegunsoye A, Oldham JM, Vij R, Husain A, Montner SM, Karwoski RA, Bartholmai BJ, Strek ME.

Sleep

Intermittent hypoxia-induced activation of endothelial cells is mediated via sympathetic activation dependent catecholamine release.

Rengul Cetin-Atalay, Angelo Y. Meliton, David Wu, Lucas M. Kimmig, Parker S. Woods, Robert B. Hamanaka, Ying-Jie Peng, Jayasri Nanduri, Nanduri Prabhakar, and Gökhan M. Mutlu. Frontiers in Physiology. 2021. DOI: 10.3389/fphys.2021.701995.

Obstructive sleep apnea phenotypes and cardiovascular risk: Is there a role for heart rate variability in risk stratification?

Carter JR, Mokhlesi B, Thomas RJ.Sleep. 2021 May 14;44(5):zsab037. doi: 10.1093/sleep/zsab037.PMID: 33988243

Optimal Continuous Positive Airway Pressure Treatment of Obstructive Sleep Apnea Reduces Daytime Resting Heart Rate in Prediabetes: A Randomized Controlled Study.

Pamidi S, Chapotot F, Wroblewski K, Whitmore H, Polonsky T, Tasali E.J Am Heart Assoc. 2020 Oct 20;9(19):e016871. doi: 10.1161/JAHA.120.016871. Epub 2020 Oct 1.PMID: 32998624

Healthcare Ethics and Allocation

Accuracy of the Sequential Organ Failure Assessment Score for In-Hospital Mortality by Race and Relevance to Crisis Standards of Care

William Dwight Miller, MD, MS; Xuan Han, MD, MS; Monica E. Peek, MD, MPH, MS; Deepshikha Charan Ashana, MD, MBA, MS; William F. Parker, MD, MS. JAMA Netw Open. 2021;4(6):e2113891. doi:10.1001/jamanetworkopen.2021.13891

Bundled Consent in US Intensive Care Units.

Espinosa ML, Tannenbaum AM, Kilaru M, Stevens J, Siegler M, Howell MD, Parker WF.Am J Crit Care. 2020 May 1;29(3):e44-e51. doi: 10.4037/ajcc2020502.PMID: 32355968

Variation in Ventilator Allocation Guidelines by US State During the Coronavirus Disease 2019 Pandemic: A Systematic Review.

Piscitello GM, Kapania EM, Miller WD, Rojas JC, Siegler M, Parker WF.JAMA Netw Open. 2020 Jun 1;3(6):e2012606. doi: 10.1001/jamanetworkopen.2020.12606.PMID: 32558916

Association of Transplant Center With Survival Benefit Among Adults Undergoing Heart Transplant in the United States.

Parker WF, Anderson AS, Gibbons RD, Garrity ER Jr, Ross LF, Huang ES, Churpek MM.JAMA. 2019 Nov 12;322(18):1789-1798. doi: 10.1001/jama.2019.15686.PMID: 31714985

Predicting Intensive Care Unit Readmission with Machine Learning Using Electronic Health Record Data.

Rojas JC, Carey KA, Edelson DP, Venable LR, Howell MD, Churpek MM.Ann Am Thorac Soc. 2018 Jul;15(7):846-853. doi: 10.1513/AnnalsATS.201710-787OC.PMID: 29787309

Accuracy of Clinicians’ Ability to Predict the Need for Intensive Care Unit Readmission.

Rojas JC, Lyons PG, Jiang T, Kilaru M, McCauley L, Picart J, Carey KA, Edelson DP, Arora VM, Churpek MM.Ann Am Thorac Soc. 2020 Jul;17(7):847-853. doi: 10.1513/AnnalsATS.201911-828OC.PMID: 32125877